Characterization of cardiolipins and their oxidation products by LC-MS analysis.
Tyurina-YY; Domingues-RM; Tyurin-VA; Maciel-E; Domingues-P; Amoscato-AA; Bayir-H; Kagan-VE
Chem Phys Lipids 2014 Apr; 179:3-10
Cardiolipins, a class of mitochondria-specific lipid molecules, is one of the most unusual and ancient phospholipids found in essentially all living species. Typical of mammalian cells is the presence of vulnerable to oxidation polyunsaturated fatty acid resides in CL molecules. The overall role and involvement of cardiolipin oxidation (CLox) products in major intracellular signaling as well as extracellular inflammatory and immune responses have been established. However, identification of individual peroxidized molecular species in the context of their ability to induce specific biological responses has not been yet achieved. This is due, at least in part, to technological difficulties in detection, identification, structural characterization and quantitation of CLox associated with their very low abundance and exquisite diversification. This dictates the need for the development of new methodologies for reliable, sensitive and selective analysis of both CLox. LC-MS-based oxidative lipidomics with high mass accuracy instrumentation as well as new software packages are promising in achieving the goals of expedited and reliable analysis of cardiolipin oxygenated species in biosamples.
Molecular-structure; Phospholipids; Mammalian-cells; Oxidation; Biological-function; Mass-spectrometry;
Author Keywords: Cardiolipin; Oxidized cardiolipin; Mass spectrometry; Lipidomics; Oxidative lipidomics
Yulia Y. Tyurina, Center for Free Radical and Antioxidant Health, Department of Environmental and Occupational Health, University of Pittsburgh, Bridgeside Point, 100 Technology Drive, Suite 350, Pittsburgh, PA 15219
Chemistry and Physics of Lipids
University of Pittsburgh at Pittsburgh