Single-walled carbon nanotubes induce fibrogenic response by promoting epithelial/fibroblast-to-myofibroblast transition: an in vitro molecular toxicity pathway based risk assessment.
He-X; Wang-L; Rojanasakul-Y
Nanotech 2014: electronics, manufacturing, environment, energy & water, technical proceedings of the 2014 NSTI nanotechnology conference and expo, June 15-18, 2014, Washington, DC. Nano Science and Technology Institute (NSTI), ed. Boca Raton, FL: CRC Press, 2014 Jun; 3:158-161
Single-walled carbon nanotubes (SWCNT) are novel material with unique electronic and mechanical properties. Several reports have shown that pulmonary exposure to SWCNT caused pulmonary fibrosis in rodents, although the molecular mechanisms of SWCNT-induced pulmonary toxicity remain largely unaddressed. Here, we report that SWCNT exert multiple effects on human lung cells. First, SWCNT induced mitochondrial inner membrane potential depolarization and generate ROS in human bronchial epithelial BEAS-2B cells, alveolar type II A549 cells, and fibroblast WI38-VA13 cells at sub-toxic doses. Second, SWCNT induced fibrogenic cytokine TGF-ß1 in BEAS-2B and macrophage RAW264.7 cells. Third, conditioned media from SWCNT-treated macrophages promoted epithelial/fibroblast- to-myofibroblast transiition, a key molecular event in the pathogenesis of lung fibrosis. This work provides a simple and rapid method for mechanism-based risk assessment of nanomaterial fibrogenicity.
Risk-analysis; Nanotechnology; Pulmonary-system-disorders; Lung-fibrosis; Lung-disorders; Respiratory-system-disorders; Fibrogenesis; Fibrogenicity; Fibrosis; Cell-cultures; Cellular-function; Cellular-reactions;
Author Keywords: nano particles; ROS; lung fibrosis
Xiaoqing He, Department of Pharmaceutical Sciences, West Virginia University, WV 26506, United States
Nano Science and Technology Institute (NSTI)
Nanotech 2014: electronics, manufacturing, environment, energy & water, technical proceedings of the 2014 NSTI nanotechnology conference and expo, June 15-18, 2014, Washington, DC