Lung epithelial cells resist influenza A infection by inducing the expression of cytochrome c oxidase VIc which is modulated by miRNA 4276.
Othumpangat-S; Noti-JD; Beezhold-DH
Virology 2014 Nov; 468-470:256-264
Influenza virus infection induces several changes in host miRNA profile, host cell death and tissue damage. Cytochrome c is a regulator of the intrinsic apoptotic pathway and is altered during viral infections. Within the first 3 h of infection with influenza virus, significant down-regulation of hsa-miRNA-4276 (miRNA-4276) is followed by a 2-fold increase in cytochrome c oxidase VIC (COX6C) mRNA was found to occur in human alveolar and bronchial epithelial cells. Expression of caspase-9 also increased within the first 3 h of infection, but subsequently decreased. Modulation of miR-4276 using mimic and inhibitor oligonucleotides showed significant down-regulation or up-regulation, respectively, of COX6C expression. Our data suggests that on initial exposure to influenza virus, host cells upregulate COX6C mRNA expression through silencing miR-4276 and repressed viral replication by inducing the apoptotic protein caspase-9. Taken together, these data suggest that miR-4276 may be an important regulator of the early stages of infection by influenza.
Ribonucleic-acids; Genes; Viral-diseases; Viral-infections; Respiratory-system-disorders; Respiratory-infections; Lung-cells; Cell-cultures; Cell-function; Cellular-reactions; Proteins; Host-resistance; Cell-metabolism; Cellular-transport-mechanism; Oxidative-phosphorylation; Cytopathology; Cell-alteration; Alveolar-cells; Cellular-respiration; Humans; Nucleotides; Cell-damage; Immune-reaction;
Author Keywords: miRNA; COX6C; Influenza virus; Lung epithelial cells
Sreekumar Othumpangat, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, MS 4020, Morgantown, WV 26505, USA
Healthcare and Social Assistance