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Domestic exposure to fungal allergenic particles determined by halogen immunoassay using subject's serum versus particles carrying three non-fungal allergens determined by allergen-specific HIA.

Authors
Sercombe-JK; Liu-Brennan-D; McKay-KO; Green-BJ; Tovey-ER
Source
Indoor Air 2014 Aug; 24(4):438-445
NIOSHTIC No.
20044925
Abstract
Studies that estimate indoor aeroallergen exposure typically measure a pre-selected limited range of allergens. In this study, inhalable aeroallergen particles were quantified using the halogen immunoassay (HIA) to determine the contribution of fungal and non-fungal aeroallergens to total allergen exposure. Bioaerosols from 39 homes of fungal-allergic subjects were sampled using inhalable fraction samplers and immunostained by HIA using resident subject's immunoglobulin E (IgE) to detect allergen-laden particles. Fungal aerosols as well as particles carrying mite, cat, and cockroach allergens were identified and enumerated by HIA. Reservoir dust-mite (Der p 1), cat (Fel d 1), and cockroach (Bla g 1) allergen concentrations were quantified by ELISA. Fungal particles that bound subject's IgE in the HIA were 1.7 (bedroom)- and 1.4 (living room)-fold more concentrated than Der p 1, Fel d 1, and Bla g 1 allergen particles combined. Predominant fungal conidia that bound IgE were derived from common environmental genera including Cladosporium and other fungi that produce amerospores. Airborne mite, cat, and cockroach allergen particle counts were not associated with reservoir concentrations determined by ELISA. This study demonstrates that inhalable fungal aerosols are the predominant aeroallergen sources in Sydney homes and should be considered in future exposure assessments.
Keywords
Indoor-environmental-quality; Air-quality; Allergic-reactions; Allergies; Allergens; Fungi; Exposure-levels; Risk-factors; Samplers; Particulates; Aerosols; Author Keywords: Aeroallergens; Bioaerosols; Allergen exposure; Airborne fungi; Fungal allergens
Contact
J. K. Sercombe, Woolcock Institute of Medical Research, Rm 630 Blackburn Bld D06, The University of Sydney, 2006, Sydney, Australia
CODEN
INAIE5
Publication Date
20140801
Document Type
Journal Article
Email Address
jason.sercombe@sydney.edu.au
Fiscal Year
2014
Identifying No.
M082014
Issue of Publication
4
ISSN
0905-6947
NIOSH Division
HELD
Priority Area
Services
Source Name
Indoor Air
State
WV
Page last reviewed: April 12, 2019
Content source: National Institute for Occupational Safety and Health Education and Information Division