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Development of a highly efficient personal sampler to collect viable bioaerosols.
Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, R01-OH-008913, 2011 Sep; :1-39
Airborne microorganisms (bioaerosols) such as viruses, allergens, bacteria, and fungal spores are almost ubiquitous in the environment. Exposure to bioaerosols in the environment may pose health risks and cause adverse health effects to the general and occupational population. Therefore, it is necessary to employ suitable methods to accurately monitor the concentration of the airborne microorganisms in the environment in order to ensure a sanitary workplace. Personal aerosol sampling is considered the most appropriate method with which to conduct health related exposure assessments. Thus, sampling bioaerosols using personal bioaerosol samplers should be a preferred method for assessing personal exposure to airborne microorganisms. This research designed, fabricated and tested a new personal bioaerosol sampler based on the rationale of a swirling cyclone incorporated a re-circulating liquid film, because bioaerosol sampling into liquid using swirling air in a cyclone features high microbial viability to the collected bioaerosols. The performance evaluation of the prototypes in a mixing chamber demonstrated that these newly developed personal bioaerosol samplers are capable of high-efficiency bioaerosol sampling with cut-off diameter (d50) equal to 0.7 um. In addition, the biological efficiency tests indicated that both samplers could permit acceptable bioaerosol survival rate for the airborne microorganisms collected. For the performance evaluations conducted in wind tunnels, the physical sampling efficiencies (aspiration efficiency, collection efficiency, and the capture efficiency) of the prototypes were tested under realistic sampling conditions. The results of the evaluation showed that the aspiration efficiency obtained from the prototypes agreed well with the ACGIH inhalable convention with slight underestimation. The collection efficiency can generally reach 80% as the aerosol size > 2 um. However, the wall loss of the prototypes was considered high especially for the aerosol size larger than 5 um which cause relatively low capture efficiency for the prototype. The test results acquired from this study enabled a full assessment of the working function of these personal bioaerosol sampler prototypes under practical sampling conditions which can offer insight into possible modifications and improvement in the future.
Microorganisms; Aerosols; Allergens; Bacteria; Fungi; Exposure-levels; Risk-factors; Environmental-exposure; Monitors; Monitoring-systems; Work-environment; Sampling; Samplers
Yung Sung Cheng, Ph.D., Lovelace Respiratory Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108
Final Grant Report
NTIS Accession No.
National Institute for Occupational Safety and Health
Lovelace Respiratory Research Institute
Page last reviewed: April 12, 2019
Content source: National Institute for Occupational Safety and Health Education and Information Division