NIOSHTIC-2 Publications Search

A sensitive LC-MS/MS method for measurement of organophosphorus pesticides and their oxygen analogs in air sampling matrices.

Armstrong-JL; Dills-RL; Yu-J; Yost-MG; Fenske-RA

J Environ Sci Health, Part B, Pestic Food Contam Agric 2014 Feb; 49(2):102-108

http://dx.doi.org/10.1080/03601234.2014.846744

20044807

A rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed for determination of levels of the organophosphorus (OP) pesticides chlorpyrifos (CPF), azinphos methyl (AZM), and their oxygen analogs chlorpyrifos-oxon (CPF-O) and azinphos methyl-oxon (AZM-O) on common active air sampling matrices. XAD-2 resin and polyurethane foam (PUF) matrices were extracted with acetonitrile containing stable-isotope labeled internal standards (ISTD). Analysis was accomplished in Multiple Reaction Monitoring (MRM) mode, and analytes in unknown samples were identified by retention time (+/-0.1 min) and qualifier ratio (+/-30% absolute) as compared to the mean of calibrants. For all compounds, calibration linearity correlation coefficients were =0.996. Limits of detection (LOD) ranged from 0.15-1.1 ng/sample for CPF, CPF-O, AZM, and AZM-O on active sampling matrices. Spiked fortification recoveries were 78-113% from XAD-2 active air sampling tubes and 71-108% from PUF active air sampling tubes. Storage stability tests also yielded recoveries ranging from 74-94% after time periods ranging from 2-10 months. The results demonstrate that LC-MS/MS is a sensitive method for determining these compounds from two different matrices at the low concentrations that can result from spray drift and long range transport in non-target areas following agricultural applications. In an inter-laboratory comparison, the limit of quantification (LOQ) for LC-MS/MS was 100 times lower than a typical gas chromatography-mass spectrometry (GC-MS) method.

Analytical-methods; Organo-phosphorus-compounds; Organo-phosphorus-pesticides; Air-sampling; Resins; Polyurethane-foams; Monitoring-systems; Sampling; Agriculture; Agricultural-chemicals; Organic-compounds; Organo-phosphorus-compounds; Pesticides; Author Keywords: Air monitoring; organophosphorus; pesticide; drift; oxon; polyurethane foam; XAD resin

Jenna L. Armstrong, Department of Environmental and Occupational Health Sciences, University of Washington School of Public Health, box 357234, Seattle, WA 98195

JPFCD2

20140201

Journal Article

jennaa@uw.edu

Cooperative Agreement

2014

Cooperative-Agreement-Number-U54-OH-007544; M082014

2

0360-1234

Agriculture, Forestry and Fishing

Journal of Environmental Science and Health. Part B: Pesticides, Food Contaminants, and Agricultural Wastes

WA

University of Washington