Development of a sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of paclobutrazol residue in wheat kernel.
Cao-Z; Zhao-H; Cui-Y; Zhang-L; Tan-G; Wang-B; Li-QX
J Agric Food Chem 2014 Feb; 62(8):1826-1831
An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with monoclonal antibody (mAb) mAb6H73C9 recognizing the plant growth regulator paclobutrazol (PBZ). The icELISA had a half-maximum inhibition concentration (IC50) and working range of approximately 8.7 and 2.0-50.4 ng/mL, respectively. Average recoveries of PBZ in the wheat (Triticum aestivum) kernel samples were between 84.3 and 118.9% with relative standard deviations between 3.9 and 14.2%. As determined by the icELISA and further confirmed by liquid chromatography-electrospray ionization quadrupole Orbitrap mass spectrometry (LC-ESI-MS) analysis, the maximum residue concentration was about 0.07 mg/kg in the kernel samples, which indicated that PBZ could transfer from PBZ- treated seedlings to the kernel samples. The correlation coefficient (R2) between icELISA and LC-ESI-MS results was 0.979, which manifested that the developed icELISA was sensitive enough for monitoring PBZ residues in wheat kernels.
Bioassays; Antibody-response; Enzymes; Enzyme-activity; Immunological-tests; Plants; Plant-cells; Agricultural-products; Agriculture; Analytical-instruments; Analytical-processes; Pesticide-residues; Liquid-chromatography; Mass-spectrometry; Electrostatic-spraying; Ionization; Food; Food-contaminants;
Author Keywords: paclobutrazol; monoclonal antibody; ELISA; LC-ESI-MS; wheat kernel
Guiyu Tan, College of Science, China Agricultural University, Beijing 100193, China
Cooperative Agreement; Agriculture
Agriculture, Forestry and Fishing
Journal of Agricultural and Food Chemistry
University of California - Davis