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Luciferase reporter cells as a platform to detect SMAD-dependent collagen production.

Lohcharoenkal W; Liu Y; Wang L; Yang Y; Rojanasakul Y

J Biosci Bioeng 2014 Dec; 118(8):732-735

https://doi.org/10.1016/j.jbiosc.2014.05.021

20044667

Aberrant collagen production can lead to many diseases such as fibrosis. Current methods of collagen detection are insensitive, time-consuming and laborious. We have developed a rapid, sensitive assay using chemiluminescence-based reporter cell system. Stable 3T3/NIH-SMAD-luciferase cells were generated for detection of collagen expression through TGF-ß signaling, a major fibrogenic pathway. We demonstrated that these reporter cells could be used as a rapid screening tool for detection of SMAD-dependent collagen production with higher sensitivity than existing assays. Flexibility of this cell-based assay in different detection platforms makes it attractive for high throughput screening of potential fibrogenic agents and drug candidates.

Screening-methods; Bioassays; Luminescence; Cytology; Diagnostic-tests; Analytical-methods; Fibrosis; Fibrogenicity; Cell-function; Cellular-reactions; Medical-screening; Biochemical-indicators; Author Keywords: Luciferase; Reporter cells; TGF-ß; Collagen; Fibrosis

Yon Rojanasakul, West Virginia University, Department of Pharmaceutical Sciences, Mary Babb Randolph Cancer Center, Morgantown, WV 26506

JBBIF6

20141201

Journal Article

yrojan@hsc.wvu.edu

2015

M072014

8

1389-1723

HELD

Manufacturing

Journal of Bioscience and Bioengineering

WV