Luciferase reporter cells as a platform to detect SMAD-dependent collagen production.
Lohcharoenkal-W; Liu-Y; Wang-L; Yang-Y; Rojanasakul-Y
J Biosci Bioeng 2014 Dec; 118(8):732-735
Aberrant collagen production can lead to many diseases such as fibrosis. Current methods of collagen detection are insensitive, time-consuming and laborious. We have developed a rapid, sensitive assay using chemiluminescence-based reporter cell system. Stable 3T3/NIH-SMAD-luciferase cells were generated for detection of collagen expression through TGF-ß signaling, a major fibrogenic pathway. We demonstrated that these reporter cells could be used as a rapid screening tool for detection of SMAD-dependent collagen production with higher sensitivity than existing assays. Flexibility of this cell-based assay in different detection platforms makes it attractive for high throughput screening of potential fibrogenic agents and drug candidates.
Screening-methods; Bioassays; Luminescence; Cytology; Diagnostic-tests; Analytical-methods; Fibrosis; Fibrogenicity; Cell-function; Cellular-reactions; Medical-screening; Biochemical-indicators;
Author Keywords: Luciferase; Reporter cells; TGF-ß; Collagen; Fibrosis
Yon Rojanasakul, West Virginia University, Department of Pharmaceutical Sciences, Mary Babb Randolph Cancer Center, Morgantown, WV 26506
Journal of Bioscience and Bioengineering