Induction of stemlike cells with fibrogenic properties by carbon nanotubes and its role in fibrogenesis.
Luanpitpong S; Wang L; Manke A; Martin KH; Ammer AG; Castranova V; Yang Y; Rojanasakul YI
Nano Lett 2014 Jun; 14(6):3110-3116
We developed a three-dimensional fibroblastic nodule model for fibrogenicity testing of nanomaterials and investigated the role of fibroblast stemlike cells (FSCs) in the fibrogenic process. We showed that carbon nanotubes (CNTs) induced fibroblastic nodule formation in primary human lung fibroblast cultures resembling the fibroblastic foci in clinical fibrosis and promoted FSCs that are highly fibrogenic and a potential driving force of fibrogenesis. This study provides a predictive 3D model and mechanistic insight on CNT fibrogenesis.
Nanotechnology; Models; Fibrogenicity; Cell-function; Cellular-function; Fibrogenesis; Humans; Men; Women; Lung; Lung-disease; Lung-function; Cell-cultures;
Author Keywords: Carbon nanotubes; lung fibrosis; stem-like fibroblasts; fibroblastic nodules; side population
Yon Rojansakul, Pharmaceutical and Pharmacological Sciences Program, West Virginia University, Morgantown, West Virginia 26506