Cellular interactions and biological responses to titanium dioxide nanoparticles in HepG2 and BEAS-2B cells: role of cell culture media.
Prasad-RY; Simmons-SO; Killius-MG; Zucker-RM; Kligerman-AD; Blackman-CF; Fry-RC; Demarini-DM
Environ Mol Mutagen 2014 May; 55(4):336-342
We showed previously that exposure of human lung cells (BEAS-2B) to TiO2 nanoparticles (nano-TiO2 ) produced micronuclei (MN) only when the final concentration of protein in the cell-culture medium was at least 1%. Nanoparticles localize in the liver; thus, we exposed human liver cells (HepG2) to nano-TiO2 and found the same requirement for MN induction. Nano-TiO2 also formed small agglomerates in medium containing as little as 1% protein and caused cellular interaction as measured by side scatter by flow cytometry and DNA damage (comet assay) in HepG2 cells. Nano-TiO2 also increased the activity of the inflammatory factor NFkB but not of AP1 in a reporter-gene HepG2 cell line. Suspension of nano-TiO2 in medium containing 0.1% protein was sufficient for induction of MN by the nanoparticles in either BEAS-2B or HepG2 cells as long the final concentration of protein in the cell-culture medium was at least 1%.
Nanotechnology; Exposure-levels; Humans; Lung-cells; Proteins; Cell-cultures; Cellular-reactions; Cellular-function; Cell-function; Pharmacology; Cytology; Metabolism; Biological-effects;
Author Keywords: micronucleus; comet assay; flow cytometry
David M. DeMarini, U.S. EPA, B105-03, RTP, NC 27711
Environmental and Molecular Mutagenesis
University of North Carolina, Chapel Hill, North Carolina