Gene expression profiles of di-n-butyl phthalate in normal human mammary epithelial cells.
Morgantown, WV: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Research Dataset RD-1001-2013-0, 2013 Jan; :dataset
Di-n-butyl phthalate is a known endocrine disruptor and has been found in increased levels in women of childbearing age. To investigate mechanisms of phthalate toxicity in normal human cells and to provide information concerning inter-individual variation and gene-environment interactions. Protocol and Methodology: Normal human mammary epithelial cell strains were obtained from discarded tissues following reduction mammoplasty. Gene transcription in each of 4 cell strains was analyzed using high-density oligonucleotide DNA microarrays (U133A, Affymetrix(TM)) and changes in the expression of selected genes were verified by real-time polymerase chain reaction. DNA microarrays were hybridized with total RNA that was collected after DBP treatment for 5 hr and 10 hr. Data Mining Tool software (Affymetrix) was used to separate genes in clusters based on their expression patterns over time. View detailed descriptions of the methods [PDF - 190KB].
Women; Cancer-rates; Chemical-hypersensitivity; Chemical-indicators; Chemical-properties; Cell-alteration; Cell-biology; Cell-growth; Cell-metabolism; Cellular-reactions; Fertility; Genes; Genetic-factors; Gene-mutation; Toxic-effects; Biomarkers; Biological-function
Ainsley Weston, NIOSH, Division of Respiratory Disease Studies, 1095 Willowdale Rd., Bldg H Rm 2910, MS H2900, Morgantown, WV 26505
Gene expression profiles of di-n-butyl phthalate in normal human mammary epithelial cells