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Hypertrophy of the rat plantar flexors following eight weeks of voluntary hindlimb resistance exercise.

Wirth O; Gregory EW; Cutlip RG; Miller GR; Metheny B
Med Sci Sports Exerc 2003 May; 35(5)(Suppl 1):S387
PURPOSE: A study was conducted to determine the effect of voluntary resistance exercise regimens on the mass of the soleus, plantaris, and gastrocnemius muscles that comprise the rat plantar flexors. METHODS: Using a custom-designed hind limb loading apparatus, 15 week old male Sprague-Dawley rats were operantly conditioned to enter a vertical tube, insert their head into a neck ring, and repeatedly lift and lower a 700-g load using a "beel raise" movement that targeted the plantar flexors. The effect of duty cycle (2 or 5 sessions per week) was studied on the mass of the soleus, plantars, and gastrocnemius muscles after 8 weeks of exercise. Age-matched sedentary rats were used as controls. Upon completion of the exercise regimen, the rats were sacrificed and the individual muscles were removed and immediately weighed. The wet-muscle weights were recorded and normalized (mg/100 g body weight). RESULTS: No significant differences in the mean muscle weights of either the soleus or plantaris were found among the groups. However, mean muscle weights of the gastrocnemius for the groups that exercised 2 sessions per week (551.93 mg/100g) or 5 sessions per week (548.91 mg/100g) were significantly greater than that of the sedentary controls (518.82 mg/100g)(p = 0.036 and p = 0.042, respectively). Mean muscle weights of the exercised groups did not differ significantly. CONCLUSION: Voluntary resistance exercise, using the present approach, resulted in an increase in gastrocnemius muscle weight of the rat in as little as eight weeks. No effect of duty cycle was found. Results suggest that the present approach is a viable model for studies of muscle hypertrophy and other physiological responses to long-term resistance exercise.
Injuries; Musculoskeletal-system; Musculoskeletal-system-disorders; Animals; Laboratory-animals; Muscle-tension; Muscle-stress; Mechanical-properties; Muscles; Muscle-tissue; Muscle-function; Injuries; Muscle-contraction; Force; Muscle-physiology; Models; Age-groups
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Medicine and Science in Sports and Exercise
Page last reviewed: March 18, 2022
Content source: National Institute for Occupational Safety and Health Education and Information Division