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Increased mechano-growth factor gene expression following stretch-shortening contraction loading: impact of glutathione and age.
Cutlip-RG; Ensey-J; Baker-BA; Hollander-MS; Kashon-ML
Med Sci Sports Exerc 2009 May; 41(5)(Suppl 1):350
Specific genes regulating multiple pathways in the adaptive response in skeletal muscle following glutathione modulation and repetitive mechanical loading are not known. PURPOSE: The purpose of this study was to characterize gene transcripts influenced by dietary supplementation with a glutathione antagonist (L-Buthionine Sulfoximine (BSO)) following chronic high-intensity mechanical loading via stretch-shortening contractions (SSCs) in young and old rats. METHODS: Left dorsiflexor muscles of young (12 weeks, N= 32) and old (30 months, N = 30), vehicle- (VEH) and BSO-treated rats were exposed 3 times per week for 4.5-weeks to a protocol of 80 maximal SSCs per exposure in vivo, while cage, age-matched rats served as controls (CON). Messenger levels for MyoD, Bax, Bcl2, Caspase-9, IL-15, IL1-b, MCP-1, TGF-b1, IL-10, mechano-growth factor (MGF), and 18s (control) genes were characterized by RT-PCR analysis following the SSC-exposure period. RESULTS: Following 4.5 weeks of SSC loading only MGF mRNA levels were significantly altered by treatment (young BSO increased versus young VEH; p < 0.05) and age (young BSO increased versus old BSO; p < 0.05). CONCLUSIONS: Even though MGF mRNA was the only transcript influenced by glutathione modulation and/or aging, further investigation is needed to evaluate changes in protein levels. However, the present study clearly demonstrates that MGF mRNA is influenced by both glutathione modulation and aging.
Genes; Musculoskeletal-system; Muscles; Muscle-physiology; Muscle-function; Animals; Laboratory-animals; Age-groups
Issue of Publication
Medicine and Science in Sports and Exercise
Page last reviewed: April 12, 2019
Content source: National Institute for Occupational Safety and Health Education and Information Division