Panel biomarker study in workers exposed to multiwalled carbon nanotube aerosol.
Fatkhutdinova LM; Khaliullin T; Kisin EK; Mustafin I; Birch EM; Kagan VE; Shvedova AA
Toxicologist 2014 Mar; 138(1):160
The aim of the study was to assess potential fibrogenic risk upon real occupational exposure to multi-walled carbon nanotubes (MWCNT) using biomarkers tested in preceding animal experiment. The study was conducted at 2 MWCNT-producing enterprises with the same reactor type. 11 workers who had more than 1 year contact with MWCNT aerosol composed the exposure group, the control group consisted of 14 people. Elemental carbon was evaluated in air samples and the CNT presence was confirmed by TEM-analysis. Blood and induced sputum samples were obtained from workers, TGF-beta1, KL-6 and osteopontin levels evaluated. To assess the relationship between MWCNT exposure and biomarker levels (age, gender, smoking have been chosen as cofounders) generalized linear models including main effects and interactions in-pairs were created. The regression coefficients confidence intervals were refined by bootstrap analysis. TWA respirable MWCNT fraction was up to 6.11 mg/m3. TEM has shown the presence of MWCNT agglomerates sized 0,5-10 mm in all air samples. It was found that exposure to MWCNT aerosol at workplaces may alter the fibrosis biomarkers in blood serum and induced sputum. The levels of TGF-beta1 in serum were dependent on exposure to MWCNTs (beta=10.5, 95%BCa=1.2-51.8), the KL-6 levels in induced sputum were significantly higher in exposure group (beta=235.9, 95%BCa=21.2-482). Osteopontin proved to be as an uninformative indicator. Data suggest that MWCNT exposure may lead to the changes in serum and induced sputum samples specific fibrogenic biomarkers content in workers. MWCNT-producing companies have to introduce control measures, as well as provide the adequate medical services organization.
Toxicology; Cell-function; Cellular-function; Cell-damage; Nanotechnology; Exposure-levels; Pulmonary-disorders; Pulmonary-function; Pulmonary-system; Pulmonary-system-disorders; Molecular-structure; Molecular-biology; Fibrosis; Exposure-levels; Laboratory-animals; Animals; Pathology; Humans; Men; Women; Biomarkers; Aerosols; Age-groups; Smoking; Air-samples; Work-environment; Workers
Mining; Manufacturing; Public Safety
The Toxicologist. Society of Toxicology 53rd Annual Meeting and ToxExpo, March 23-27, 2014, Phonex, Arizona