Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers.
Wu-F; Liu-J; Qiu-Y-L; Wang-W; Zhu-S-M; Sun-P; Miao-W-B; Li-Y-L; Brandt-Rauf-PW; Xia-Z-L
Int J Occup Med Environ Health 2013 Mar; 26(1):173-182
OBJECTIVE: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). MATERIALS AND METHODS: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specific PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups. RESULTS: MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a significant difference in MGMT methylation between the two groups (p < 0.05). CONCLUSIONS: We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study.
DNA-damage; Chromosome-damage; Genetics; Metabolism; Drug-interaction; Enzymes; Proteins; Monomers; Bioassays; Blood-cells; Lymphocytes; Gases; Ethylenes; Chlorides; Genes; Polymerization; Humans; Men; Women; Tumor-inhibition; Exposure-assessment;
Author Keywords: Vinyl chloride monomer; Chromosome damage; MGMT; hMLH1; DNA methylation
Z-L Xia, Department of Occupational Health and Toxicology, School of Public Health, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China
International Journal of Occupational Medicine and Environmental Health
University of Illinois at Chicago