Lung tumor promotion by chromium-containing welding particulate matter in a mouse model.
Zeidler-Erdely PC; Meighan TG; Erdely A; Battelli LA; Kashon ML; Keane M; Antonini JM
Part Fibre Toxicol 2013 Sep; 10:45
Background: Epidemiology suggests that occupational exposure to welding particulate matter (PM) may increase lung cancer risk. However, animal studies are lacking to conclusively link welding with an increased risk. PM derived from stainless steel (SS) welding contains carcinogenic metals such as hexavalent chromium and nickel. We hypothesized that welding PM may act as a tumor promoter and increase lung tumor multiplicity in vivo. Therefore, the capacity of chromium-containing gas metal arc (GMA)-SS welding PM to promote lung tumors was evaluated using a two-stage (initiation-promotion) model in lung tumor susceptible A/J mice. Methods: Male mice (n = 28-30/group) were treated either with the initiator 3-methylcholanthrene (MCA;10 ug/g; IP) or vehicle (corn oil) followed by 5 weekly pharyngeal aspirations of GMA-SS (340 or 680 ug/exposure) or PBS. Lung tumors were enumerated at 30 weeks post-initiation. Results: MCA initiation followed by GMA-SS welding PM exposure promoted tumor multiplicity in both the low (12.1+/- 1.5 tumors/mouse) and high (14.0 +/- 1.8 tumors/mouse) exposure groups significantly above MCA/sham (4.77 +/- 0.7 tumors/mouse; p = 0.0001). Multiplicity was also highly significant (p < 0.004) across all individual lung regions of GMA-SS-exposed mice. No exposure effects were found in the corn oil groups at 30 weeks. Histopathology confirmed the gross findings and revealed increased inflammation and a greater number of malignant lesions in the MCA/welding PM-exposed groups. Conclusions: GMA-SS welding PM acts as a lung tumor promoter in vivo. Thus, this study provides animal evidence to support the epidemiological data that show welders have an increased lung cancer risk.
Laboratories; Laboratory-animals; Animals; Lung; Welders; Welding; Epidemiology; Exposure-levels; Risk-factors; Parasiticides; Cancer; Carcinogens; Metals; Metallic-compounds; Tumors; Chromium-compounds; Exposure-levels; Welding;
Author Keywords: A/J mouse; Cancer; Chromium; Nickel; Welding
Patti C Zeidler-Erdely, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road MS L2015, Morgantown, WV 26505
18540-29-9; 7440-02-0; 56-49-5
Particle and Fibre Toxicology