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Diacetyl increases sensory innervation and substance P production in rat trachea.
Goravanahally-MP; Hubbs-AF; Fedan-JS; Kashon-ML; Battelli-LA; Mercer-RR; Goldsmith-WT; Jackson-MC; Cumpston-A; Frazer-DG; Dey-RD
Toxicol Pathol 2014 Apr; 42(3):582-590
Inhalation of diacetyl, a butter flavoring, causes airway responses potentially mediated by sensory nerves. This study examines diacetyl-induced changes in sensory nerves of tracheal epithelium. Rats (n = 6/group) inhaled 0-, 25-, 249-, or 346-ppm diacetyl for 6 hr. Tracheas and vagal ganglia were removed 1-day postexposure and labeled for substance P (SP) or protein gene product 9.5 (PGP9.5). Vagal ganglia neurons projecting to airway epithelium were identified by axonal transport of fluorescent microspheres intratracheally instilled 14 days before diacetyl inhalation. End points were SP and PGP9.5 nerve fiber density (NFD) in tracheal epithelium and SP-positive neurons projecting to the trachea. PGP9.5-immunoreactive NFD decreased in foci with denuded epithelium, suggesting loss of airway sensory innervation. However, in the intact epithelium adjacent to denuded foci, SP-immunoreactive NFD increased from 0.01 +/- 0.002 in controls to 0.05 +/- 0.01 after exposure to 346-ppm diacetyl. In vagal ganglia, SP-positive airway neurons increased from 3.3 +/- 3.0% in controls to 25.5 +/- 6.6% after inhaling 346-ppm diacetyl. Thus, diacetyl inhalation increases SP levels in sensory nerves of airway epithelium. Because SP release in airways promotes inflammation and activation of sensory nerves mediates reflexes, neural changes may contribute to flavorings-related lung disease pathogenesis.
Food-additives; Laboratory-animals; Animal-studies; Animals; Nerves; Nerve-tissue; Nerve-fibers; Nervous-system-function; Pulmonary-system; Author Keywords: flavorings; inhalation toxicity; airway epithelium; airway innervation; airway inflammation; cough
Richard Dey, Department of Neurobiology and Anatomy, P.O. Box 9128, West Virginia University, Morgantown, WV 26506
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Page last reviewed: April 12, 2019
Content source: National Institute for Occupational Safety and Health Education and Information Division