Neoplastic-like transformation effect of single-walled and multi-walled-carbon nanotubes compared to asbestos on human lung small airway epithelial cells.
Wang L; Stueckle TA; Mishra A; Derk R; Meighan T; Castranova V; Rojanasakul Y
Nanotoxicology 2013 Aug; 8(5):485-507
Accumulating evidence indicates that carbon nanotubes (CNTs) are biopersistent and can cause lung damage. With similar fibrous morphology and mode of exposure to asbestos, a known human carcinogen, growing concern has arisen for elevated risk of CNT-induced lung carcinogenesis; however, relatively little is known about the long-term carcinogenic effect of CNT. Neoplastic transformation is a key early event leading to carcinogenesis. We studied the ability of single- and multi-walled CNTs to induce neoplastic transformation of human lung epithelial cells compared to asbestos. Long-term (6 month) exposure of the cells to occupationally relevant concentrations of CNT in culture caused a neoplastic-like transformation phenotype as demonstrated by increased cell proliferation, anchorage-independent growth, invasion and angiogenesis. Whole genome expression signature and protein expression analyses showed that single- and multi-walled CNTs shared similar signaling signatures which were distinct from asbestos. These results provide novel toxicogenomic information and suggest distinct particle-associated mechanisms of neoplasia promotion induced by CNTs and asbestos.
Nanotechnology; Lung; Lung-disease; Lung-function; Fibrosis; Fibrous-bodies; Exposure-levels; Risk-factors; Carcinogens; Cell-biology; Cell-function; Cellular-function; Humans; Men; Women;
Author Keywords: lung; neoplastic transformation; invasion; genome expression; microarray
Liying Wang, HELD/PPRB, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505