Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways.
Zhao J; Bowman L; Magaye R; Leonard SS; Castranova V; Ding M
Int J Oncol 2013 Apr; :42(4):1349-1359
In this study, apoptosis and related signaling induced by WC-Co nanoparticles were investigated in JB6 cells and rat lung macrophages. Electron spin resonance (ESR) and fluorescent staining indicated that both WC-Co nanoparticles and fine particles stimulated reactive oxygen species (ROS) generation. Catalase exhibited an inhibitory effect on WC-Co nanoparticle-induced ROS as well as mitochondrial membrane permeability damage. Further study indicated that WC-Co nanoparticles elicited higher cytotoxicity and apoptotic induction than fine particles. Western blot analysis showed activation of proapoptotic factors including Fas, Fas-associated protein with death domain (FADD), caspase 3, 8 and 9, BID and BAX. In addition, both cytochrome c and apoptosis-inducing factor (AIF) were upregulated and released from mitochondria to the cytoplasm. Our findings demonstrate that, on a mass basis, WC-Co nanoparticles exhibit higher cytotoxicity and apoptotic induction than fine particles. Apoptosis induced by WC-Co nanoparticles and fine particles involves both extrinsic and intrinsic apoptosis pathways.
Nanotechnology; Animals; Laboratory-animals; Lung; Particulates; Cytotoxicity;
Author Keywords: JB6 cells; rat lung macrophages; cytochrome c; apoptosis-inducing factor; mitochondria
Jinshun Zhao, Ningbo Univ, Zhejiang Prov Key Lab Pathol & Physiol Technol, Sch Med, Dept Prevent Med, 818 Fengua Rd, Ningbo 315211, Zhejiang, Peoples R China
International Journal of Oncology