Differential expression kinetics of miRNA involved in allergic chemical sensitization following dermal exposure in a murine model.
Anderson S; Beezhold K; Lukomska E; Richardson J; Long CM; Anderson K; Franko J; Meade BJ; Beezhold DH
Toxicologist 2013 Mar; 132(1):90
Allergic disease is an important occupational health concern with work related asthma and allergic contact dermatitis among the most frequently diagnosed occupational illnesses. The development of rapid and sensitive methods for hazard identification of the responsible agents is critical. MicroRNAs (miRNAs) are small noncoding RNAs 20-22 nucleotides long whose primary function is to regulate gene expression by functioning as endogenous inhibitors of protein translation. Allergic disease is characterized by an imbalance between Th1 and Th2 cytokines; however the role of posttranscriptional mechanisms like the ones regulated by miRNAs is just starting to be explored. These studies describe the kinetics of miRNA expression during the sensitization phase of an allergic response following dermal exposure to prototypical chemical sensitizers in a mouse model. Using microarray and other data, six miRNAs were identified for further analysis with RT-PCR including mi-21, 22, 210, 155, 133a, and 27b. These data demonstrate that miRNAs may have a central role early in the allergic response focused on establishing the fine balance of Th1 verses Th2 responses to chemical sensitizers. Identification of unique miRNA expression profiles may help to elicit the mechanisms by which exposure to sensitizing chemicals induce immune cell activation and can potentially help to identify biomarkers for new treatments and preventions.
Toxicology; Bronchial-asthma; Allergic-dermatitis; Laboratory-techniques; Exposure-assessment; Exposure-levels; Pulmonary-function; Pulmonary-system-disorders; Lung-function; Dose-response; Lung-disorders; Lung-irritants; Respiratory-system-disorders; Skin-exposure; Allergic-reactions; Nucleotides; Genes; Protein-biochemistry; Proteins; Cytochemistry; Kinetics; Sensitization; Analytical-models
The Toxicologist. Society of Toxicology 52nd Annual Meeting and ToxExpo, March 10-14, 2013, San Antonio, Texas