1-Hydroxypyrene as a biomarker for assessing the effects of exposure to polycyclic aromatic hydrocarbons on semen quality and sperm DNA integrity.
Jeng HA; Pan C-H; Chao M-R
J Environ Sci Health. Part A, Tox Hazard Subst Environ Eng 2013 Jan; 48(2):152-158
A cross-sectional study was conducted to assess whether urinary 1-hydroxypyrene (1-OHP) could serve as a biomarker to assess the effect of PAHs on cellular and molecular changes of sperm. Urine and semen samples were collected from a total of 65 healthy coke oven workers. Sperm quality parameters (concentration, motility, vitality, and morphology) and semen integrity (DNA fragmentation, 8-oxodGuo, bulky DNA adducts) were analyzed. Sixteen (16) targeted PAHs at the personal breathing zone area were monitored and quantified. Results showed that urinary 1-OHP positively correlated with measured levels of 16 targeted PAHs. Urinary 1-OHP did not significantly correlate with semen quality; however, PAHs with heavy molecular weight, e.g., benzo(g,h,i)perylene and benzo(k)fluoranthene, negatively correlated with morphology and motility of sperms (p = 0.02 and 0.002, p = 0.04 and 0.04, respectively). Urinary 1-OHP positively correlated with the level of 8-oxodGuo and bulky DNA adducts, but not DNA fragmentation. Urinary 1-OHP was a suitable biomarker for an estimate of biologically effective doses of PAH exposure. However, urinary 1-OHP may not be sufficient as a biomarker to assess both cellular and molecular changes of sperm induced by PAHs.
Urinalysis; Urine-chemistry; Biomarkers; Cell-alteration; Cell-function; Cellular-function; Cellular-reactions; Analytical-processes; Humans; Men; Reproductive-system; Reproductive-system-disorders; Morphology; Molecular-biology; Molecular-structure; Metallurgy; Exposure-levels; Chromatographic-analysis; Age-groups; Pyrenes; Metabolism; Pathology; DNA-adducts; DNA-damage; Hydroxy-compounds;
Author Keywords: 1-Hydroxypyrene; semen quality; DNA integrity; biomarker; 8-oxodGuo; DNA fragmentation
Hueiwang Anna Jeng, School of Community and Environmental Health, College of Health Sciences, Old Dominion University, 4608 Hampton Boulevard, Health Sciences Building, Room 3140, Norfolk,VA USA
Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering
Old Dominion University, Norfolk, Virginia