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The group A streptococcal collagen-like protein-1, Scl1, mediates biofilm formation by targeting the extra domain A-containing variant of cellular fibronectin expressed in wounded tissue.

Authors
Oliver-Kozup-H; Martin-KH; Schwegler-Berry-D; Green-BJ; Betts-C; Shinde-AV; Van De Water-L
Source
Mol Microbiol 2013 Feb; 87(3):672-689
Link
http://dx.doi.org/10.1111/mmi.12125
NIOSHTIC No.
20042232
Abstract
Wounds are known to serve as portals of entry for group A Streptococcus (GAS). Subsequent tissue colonization is mediated by interactions between GAS surface proteins and host extracellular matrix components. We recently reported that the streptococcal collagen-like protein-1, Scl1, selectively binds the cellular form of fibronectin (cFn) and also contributes to GAS biofilm formation on abiotic surfaces. One structural feature of cFn, which is predominantly expressed in response to tissue injury, is the presence of a spliced variant containing extra domain A (EDA/EIIIA). We now report that GAS biofilm formation is mediated by the Scl1 interaction with EDA-containing cFn. Recombinant Scl1 proteins that bound cFn also bound recombinant EDA within the C-C' loop region recognized by the a9ß1 integrin. The extracellular 2-D matrix derived from human dermal fibroblasts supports GAS adherence and biofilm formation. Altogether, this work identifies and characterizes a novel molecular mechanism by which GAS utilizes Scl1 to specifically target an extracellular matrix component that is predominantly expressed at the site of injury in order to secure host tissue colonization.
Keywords
Injuries; Bacteria; Proteins; Cellular-reactions; Skin; Skin-infections; Molecular-biology; Tissue-culture; Microorganisms; Bacterial-infections; Bacteriology; Bacterial-cultures; In-vitro-studies
Contact
Slawomir Lukomski, Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, WV 26506
CODEN
MOMIEE
Publication Date
20130201
Document Type
Journal Article
Email Address
slukomski@hsc.wvu.edu
Fiscal Year
2013
Identifying No.
B20130306
Issue of Publication
3
ISSN
0950-382X
NIOSH Division
HELD
Priority Area
Services
Source Name
Molecular Microbiology
State
WV; NY
Page last reviewed: April 12, 2019
Content source: National Institute for Occupational Safety and Health Education and Information Division