Vitamin D treatment modulates organic dust-induced cellular and airway inflammatory consequences.
Golden-GA; Wyatt-TA; Romberger-DJ; Reiff-D; McCaskill-M; Bauer-C; Gleason-AM; Poole-JA
J Biochem Mol Toxicol 2013 Jan; 27(1):77-86
Exposure to organic dusts elicits airway inflammatory diseases. Vitamin D recently has been associated with various airway inflammatory diseases, but its role in agricultural organic dust exposures is unknown. This study investigated whether vitamin D reduces organic dust-induced inflammatory outcomes in cell culture and animal models. Organic dust extracts obtained from swine confinement facilities induced neutrophil chemokine production (human IL-8, murine CXCL1/CXCL2). Neutrophil chemokine induction was reduced in human blood monocytes, human bronchial epithelial cells, and murine lung slices pretreated with 1,25-(OH)(2) D(3) . Intranasal inhalation of organic dust extract induced neutrophil influx, and CXCL1/CXCL2 release was also decreased in mice fed a relatively high vitamin D diet as compared to mice fed a low vitamin D diet. These findings were associated with reduced tracheal epithelial cell PKCa and PKCe activity and whole lung TLR2 and TLR4 gene expression. Collectively, vitamin D plays a role in modulating organic dust-induced airway inflammatory outcomes.
Respiratory-system-disorders; Airway-obstruction; Airway-resistance; Breathing; Organic-dusts; Vitamins; Immune-reaction; Lung-cells; Lung-disorders; Lung-irritants; Pulmonary-function; Pulmonary-system-disorders; Cell-cultures; Agricultural-workers; Agriculture; Dust-exposure; Dust-inhalation; Laboratory-animals; Neutrophils
Jill A. Poole, Pulmonary, Critical Care, Sleep and Allergy Division, Department of Medicine, University of Nebraska Medical Center, Omaha, NE 68198-5300, USA
Agriculture, Forestry and Fishing
Journal of Biochemical and Molecular Toxicology
University of Nebraska Medical Center - Omaha