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Induction of apoptosis by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both 'extrinsic' and 'intrinsic' apoptotic pathways.

Ding M; Zhao J; Bowman L; Leonard S; Castranova V

FASEB J 2012 Apr; 26(Meeting Abstracts):798.26

https://www.fasebj.org/doi/10.1096/fasebj.26.1_supplement.798.26

20041980

Tungsten Carbide-Cobalt (WC-Co) nanoparticle composites have wide applications because of their hardness and toughness. In this study, apoptosis and related signaling induced by WC-Co were investigated. Electron spin resonance (ESR) and fluorescent staining indicated that both WC-Co nano- and fine particles stimulated reactive oxygen species (ROS) generation. Catalase inhibit WC-Co-induced ROS as well as mitochondrial membrane permeability damage, indicating that H2O2 may play an important role in the cytotoxicity induced by WC-Co. Further studies indicated that WC-Co nanoparticles elicited a higher cytotoxicity and apoptotic induction than fine particles. Western-blot analysis showed an activation of proapoptotic factors including Fas, FADD, caspase 3, 8 and 9, BID, and BAX. Lamin A and beta-actin were cleaved. Interestingly, WC-Co particles also induced Bcl-2, an anti-apoptotic factor, up-regulation. In addition, both cytochrome c and AIF were up-regulated and released from mitochondria to the cytoplasm. The results demonstrate that apoptosis induced by WC-Co involve both 'extrinsic' and 'intrinsic' apoptotic pathways and nanoparticles exhibit higher cytotoxicity and apoptotic induction than fine particles.

Tungsten-compounds; Nanotechnology; Particulates; Particulate-dust; Particle-aerodynamics; Cytotoxicity

FAJOEC

7440-33-7; 11130-73-7; 7440-48-4

20120401

Abstract

2012

B20130124

0892-6638

HELD

The FASEB Journal

WV