New perspectives for in vitro risk assessment of multiwalled carbon nanotubes: application of coculture and bioinformatics.
Snyder-Talkington-BN; Qian-Y; Castranova-V; Guo-NL
J Toxicol Environ Health, B 2012 Nov; 15(7):468-492
Nanotechnology is a rapidly expanding field with wide application for industrial and medical use; therefore, understanding the toxicity of engineered nanomaterials is critical for their commercialization. While short-term in vivo studies have been performed to understand the toxicity profile of various nanomaterials, there is a current effort to shift toxicological testing from in vivo observational models to predictive and high-throughput in vitro models. However, conventional monoculture results of nanoparticle exposure are often disparate and not predictive of in vivo toxic effects. A coculture system of multiple cell types allows for cross-talk between cells and better mimics the in vivo environment. This review proposes that advanced coculture models, combined with integrated analysis of genome-wide in vivo and in vitro toxicogenomic data, may lead to development of predictive multigene expression-based models to better determine toxicity profiles of nanomaterials and consequent potential human health risk due to exposure to these compounds.
Nanotechnology; Industrial-environment; Toxic-materials; In-vivo-studies; In-vitro-study; Models; Exposure-levels; Toxic-effects; Cell-cultures; Analytical-processes; Risk-factors
Yong Qian, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505-2888
Journal of Toxicology and Environmental Health, Part B: Critical Reviews