Kerosene cleaning disturbs the barrier function of the skin and alters the organ distribution of BAP applied topically in mice.
Talaska G; LaDow C; Schumann B; Luce N; Warshawsky D; Pickens B; Hoath S
American Industrial Hygiene Conference and Exposition, June 2-7, 2001, New Orleans, Louisiana. Fairfax, VA: American Industrial Hygiene Association, 2001 Jun; :40
Kerosene cleaning following treatment with used gasoline engine oil, a complex mixture containing polycyclic aromatic hydrocarbons, increased the relative and absolute levels of DNA adducts in the lungs of mice relative to animals treated with the used oil alone. To determine the mechanism for the effect, we topically treated animals with 3HBAP( 100 nMol in 25 ul acetone) and washed Yl the animals with 25 ul kerosene 1 hour after the carcinogen application. Groups of 4 mice were sacrificed at 1, 4, 12, 24, 48 and 72 hours after the BAP treatment and lung, liver and skin were harvested. The levels of radioactivity were determined in each tissue and DNA was also isolated for determination of BAP-DNA adduct levels. The fraction of the radiolabel which remained in the skin of the animals treated with BAP but not washed with kerosene was 99.9 (std = 0.1), 98.1 (1.0), 91.6 (4.6), 99 (0.5), 98.6 (0.6) and 86.9% (7.4), respectively for each time point. In the animals that were washed with kerosene the comparative levels were 99.5 (0.1), 96.7 (1.3), 97.6 (1.0), 77.3 (9.2), 64.6 (9.1) and 69% (13.5). respectively. The relative fractions at 24 and 48, but not 72 hours were statistically significantly different at the 0.05 level between the two groups. At these same time points the fraction distribution have found that kerosene increases the transdermal water loss following both single and multiple treatments. These data suggest that even a single small volume treatment with kerosene compromises the protective capabilities of the skin and enhances absorption topical carcinogens and alters their internal distribution.
Exposure-levels; Skin; Skin-exposure; Gas-mixtures; Hydrocarbons; Lung-tissue; Lung; Laboratory-animals; Oils; Carcinogens; Mutagenicity
American Industrial Hygiene Conference and Exposition, June 2-7, 2001, New Orleans, Louisiana
University of Cincinnati, Cincinnati, Ohio