Use of photoaffinity labels as P2-purinoceptor antagonists.
Fedan-JS; Hogaboom-GK; O'Donnell-JP; Westfall-DP
Methods Used in Adenosine Research (Methods in Pharmacology. Volume 6). Paton DM, ed., New York: Plenum Press, 1985 Jan; 6:279-292
There are both disadvantages and advantages to the use of the photoaffinity label ANAPP3 in the study of P2 receptors. The most obvious disadvantage is that ANAPP3 cannot be used as an irreversible antagonist in vivo, because there is no mechanism, at present, to irradiate the compound under these conditions. Related to this is the potential difficulty of photolyzing ANAPP3 in vitro if relatively thick tissue preparations are being investigated. The novelty of dealing with photoaffinity compounds, as well as the unusual apparatus, such as the lamps that are required, may seem to be a disadvantage as well. In spite of these difficulties, however, ANAPP3 is an important tool for investigators interested in the pharmacology and physiology of adenine nucleotides and nucleosides. ANAPP3 has the advantage of being the only specific antagonist of responses mediated through P2 receptors. Thus, the compound is of considerable value in probing the potential physiological role of ATP and in helping to distinguish between responses mediated by ATP and by adenosine.
In-vivo-studies; In-vitro-studies; Analytical-processes; Enzyme-activity; Enzymes
Book or book chapter
Methods Used in Adenosine Research (Methods in Pharmacology. Volume 6)