Polymorphisms in the CD14 gene associated with pulmonary function in farmers.
LeVan-TD; Von Essen-S; Romberger-DJ; Lambert-GP; Martinez-FD; Vasquez-MM; Merchant-JA
Am J Respir Crit Care Med 2005 Apr; 171(7):773-779
RATIONALE AND OBJECTIVES: Farmers experience airway obstruction, which may be attributable in part to endotoxin inhalation. CD14 is a receptor for endotoxin. MATERIALS AND METHODS: Based on our findings of increased circulating CD14 associated with the CD14/-159 T allele, we hypothesized that carriers of this allele would have decreased lung function among endotoxin-exposed individuals. CD14/-159TT farmers (n = 19) had significantly lower lung function as measured by FEV1 (p = 0.028) and mean forced expiratory flow during the middle half of the FVC (FEF25-75) (p = 0.05) compared with farmers with the C allele (n = 78). Also, farmers with the CD14/-1619GG genotype (n =11) were associated with lower lung function (FEV1, p = 0.008; FEF25-75, p = 0.009) compared with farmers with the A allele (n = 86). RESULTS: No association between CD14/-550 and lung function was observed (FEV1, p = 0.32; FEF25-75, p = 0.11). Increased prevalence of wheezing was reported in farmers homozygous for CD14/-159T (p = 0.013) or CD14/-1619G (p = 0.019) compared with farmers with the CC or AA genotype, respectively. No association was found between TLR4/Asp299Gly and lung function or wheeze. CONCLUSION: We conclude that the CD14/-159 or CD14/-1619 loci may play a role in modulating lung function and wheeze among agricultural workers.
Farmers; Pulmonary-function; Pulmonary-system; Pulmonary-disorders; Respiratory-system-disorders; Respiration; Lung-function; Lung-disorders; Humans; Men; Women; Endotoxins;
Author Keywords: endotoxin; farming; lipopolysaccharide; occupational exposure; wheeze
Susanna Von Essen, M.D., M.P.H., Department of Internal Medicine, Pulmonary and Critical Care Medicine Section, University of Nebraska, Omaha, NE 68198-5300
American Journal of Respiratory and Critical Care Medicine
University of Iowa