Impaired clearance and enhanced pulmonary inflammatory/fibrotic response to carbon nanotubes in myeloperoxidase-deficient mice.
Shvedova-AA; Kapralov-AA; Feng-WH; Kisin-ER; Murray-AR; Mercer-RR; St. Croix-CM; Lang-MA; Watkins-SC; Konduru-NV; Allen-BL; Conroy-J; Kotchey-GP; Mohamed-BM; Mead-AD; Volkov-Y; Star-A; Fadeel-B; Kagan-VE
PLoS One 2012 Mar; 7(3):e30923
Advancement of biomedical applications of carbonaceous nanomaterials is hampered by their biopersistence and proinflammatory action in vivo. Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. Our results provide direct evidence for the participation of MPO - one of the key-orchestrators of inflammatory response - in the in vivo pulmonary oxidative biodegradation of SWCNT and suggest new ways to control the biopersistence of nanomaterials through genetic or pharmacological manipulations.
Pulmonary-system; Pulmonary-system-disorders; Pulmonary-function-tests; Pulmonary-function; Fibrosis; Oxidative-processes; Stress; Mesothelial-cells; Carcinogens; Laboratory-animals; Cell-biology; Cell-cultures; Cell-function; Animals; Animal-studies; Nanotechnology
Public Library of Science One
University of Pittsburgh at Pittsburgh