Immunologic, spectrophotometric and nucleic acid based methods for the detection and quantification of airborne pollen.
Rittenour-WR; Hamilton-RG; Beezhold-DH; Green-BJ
J Immunol Methods 2012 Sep; 383(1-2):47-53
Microscopic identification of pollen morphological phenotypes has been the traditional method used to identify and quantify pollen collected by air monitoring stations worldwide. Although this method has enabled a semi-standardized approach for the assessment of pollen exposure, limitations including labor intensiveness, required expertise, examiner bias, and the inability to differentiate species, genera, and in some cases families have limited data derived from the these stations. Recent advances in chemical, biochemical and molecular detection methods have provided standardized alternatives to this microscopic approach. In this review, we examine the applicability of alternative methodologies, in particular nucleic acid based assays involving the quantitative polymerase chain reaction, for the standardized detection of airborne pollen.
Air-contamination; Airborne-particles; Allergens; Immunological-tests; Bioassays; Analytical-instruments; Microscopic-analysis; Spectrophotofluorometry; Nucleic-acids; Quantitative-analysis; Morphology; Air-monitoring; Analytical-methods; Standards;
Author Keywords: Allergen; Immunoassay; Pollen; Polymerase chain reaction
Donald H. Beezhold, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute of Occupational Safety and Health, CDC, 1095 Willowdale Road, M.S. 4020, Morgantown, WV 26505, USA
Journal of Immunological Methods