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Molecular methods for bioaerosol characterization.
Summerbell-RC; Green-BJ; Corr-D; Scott-JA
Microorganisms in home and indoor work environments: diversity, health impacts, investigation and control, second edition. Flannigan B, Samson RA, Miller JD, eds. Boca Raton, FL: CRC Press, 2011 Jan; :247-264
Since the work of Maddox (1857), Cunningham (1873), Miquel (1883) and othes in the latter part of the 19th century, the detection and classification of bioaerosols has been dominated by methods based on culture and microscopy. The invention of polymerase chain reaction (PCR) in the 1980s transformed all of biology, including the study of bioaerosols. At first. this technique for amplifying genes was primarily used for single species, sampled as tissue specimens or as living cultures (Sontakke et al. 2009). Very rapidly, however, scientists realized that by using conservative gene regions as primer areas, they could amplify an indefinitely large diversity of different species from complex mixtures of organisms without a priori knowledge of their DNA sequences (Nocker et al.2007). Thus, a very powerful techhnique for environmental investigation had been made available. By adjusting the primers used, amplification could be targeted at groups of organisms with any level of specificity: classes, orders, families, genera, species or strains/individuals. In cases where there was strategic advantage in limiting the diversity elucidated from complex environmental samples, specific primers could be targeted at one, several, or multiple species (Haugland etal. 2004). These primers could then be used as a battery to investigate single species or to simultaneously look for two to four species at a time, as is made possible in some quantitative 'real time' PeR techniques.
Microorganisms; Microbiology; Air-contamination; Airborne-particles; Dispersion; Bacteria; Pathogenicity; Respiratory-irritants; Analytical-processes; Analytical-methods; Biological-factors; Genes; Tissue-culture
Flannigan-B; Samson-RA; Miller-JD
Microorganisms in home and indoor work environments: diversity, health impacts, investigation and control, second edition
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Content source: National Institute for Occupational Safety and Health Education and Information Division