Mass-spectrometric characterization of peroxidized and hydrolyzed lipids in plasma and dendritic cells of tumor-bearing animals.
Tyurin-VA; Cao-W; Tyurina-YY; Gabrilovich-DI; Kagan-VE
Biochem Biophys Res Commun 2011 Sep; 413(1):149-153
Dendritic cells are the most potent antigen presenting cells responsible for the development of immune responses in cancer. However, it is known that the function of dendritic cells in tumor-bearing hosts is severely compromised. Our previous studies demonstrated that the defects in dendritic cell function are due, to a large extent, to the accumulation of high amounts of lipids--predominantly triglycerides--in a substantial proportion of dendritic cells in tumor-bearing mice and patients with cancer. The dendritic cells accumulation of lipids is likely associated with their up-regulation of a scavenger receptor A. This receptor is primarily responsible for uptake of modified lipids. Here, by using different versions of liquid chromatography-mass spectrometry, we identified several molecular species of oxygenated lipids in plasma of tumor-bearing animals that may be responsible for their uptake and accumulation by dendritic cells via scavenger receptor A-dependent pathway--the effect that may be associated with the loss of dendritic cell's immune surveillance function in cancer.
Cell-alteration; Cellular-function; Cellular-reactions; Cancer; Antigens; Immune-reaction; Immune-system; Tumors; Glycerides; Lipids; Animal-studies; Laboratory-animals; Laboratory-testing; Cellular-uptake; Chromatographic-analysis; Mass-spectrometry; Molecular-structure;
Author Keywords: Cancer; Tumor-bearing mice; Plasma; Dendritic cells; Scavenger receptor A; Lipid uptake; Oxygenated fatty acids
Valerian E. Kagan, Department of Environmental and Occupational Health, University of Pittsburgh, Bridgeside Point, 100 Technology Drive, Room 330, BRIDG, Pittsburgh, PA 15219-3130, USA
Biochemical and Biophysical Research Communications
University of Pittsburgh at Pittsburgh