Urinary 8-hydroxy-2'-deoxyguanosine as a biomarker of oxidative DNA damage induced by ambient pollution in the Normative Aging Study.
Ren-C; Fang-S; Wright-RO; Suh-H; Schwartz-J
Occup Environ Med 2011 Aug; 68(8):562-569
Background: Studies show that exposure to air pollution damages human health, but the mechanisms are not fully understood. One suggested pathway is via oxidative stress. Objectives: This study examines associations between exposure to air pollution and oxidative DNA damage, as indicated by urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) concentrations in ageing participants during 2006-2008. Methods: We fit linear regression models to examine associations between air pollutants and 8-OHdG adjusting for potential confounders. Results: 8-OHdG was significantly associated with ambient particulate matter =2.5 Ám in aerodynamic diameter (PM2.5), nitrogen dioxide (NO2), maximal 1 h ozone (O3), sulphate (SO42-) and organic carbon (OC), but not with black carbon (BC), carbon monoxide (CO), the number of particles (PN) or elemental carbon (EC). Effects were more apparent with multi-week averages of exposures. Per IQR increases in 21-day averages of PM2.5, PN, BC, EC, OC, CO, SO42-, NO2 and maximal 1 h O3 were associated with 30.8% (95% CI 9.3% to 52.2%), -13.1% (95% CI -41.7% to 15.5%), 3.0% (95% CI -19.8% to 25.8%), 5.3% (95% CI -23.6% to 34.2%), 24.4% (95% CI 1.8% to 47.1%), -2.0% (95% CI -12.4% to 8.3%), 29.8% (95% CI 6.3% to 53.3%), 32.2% (95% CI 7.4% to 56.9%) and 47.7% (95% CI 3.6% to 91.7%) changes in 8-OHdG, respectively. Conclusions: This study suggests that ageing participants experienced an increased risk of developing oxidative DNA injury after exposure to secondary, but not primary, ambient pollutants.
Age-factors; Airborne-particles; Analytical-methods; Epidemiology; Exposure-assessment; Exposure-levels; Exposure-methods; Molecular-biology; Oxidation; Oxidative-metabolism; Oxidative-processes; Particle-aerodynamics; Particulates; Particulates; Pollutants; Statistical-analysis; Urine-chemistry
Cizao Ren, Department of Environmental Health, Exposure, Epidemiology and Risk Program, Harvard School of Public Health, Landmark Center, West, Suite 415, PO Box 15677, 401 Park Dr., Boston, MA 02215, USA
10102-44-0; 1333-86-4; 630-08-0; 7440-44-0
Occupational and Environmental Medicine
Harvard School of Public Health