Assessment of exposure to PACs in asphalt workers: measurement of urinary PACs and their metabolites with an ELISA kit.
Smith-JP; Biagini-RE; Johnson-BC; Olsen-LD; Mackenzie-BA; Robertson-SA; Sammons-DL; Striley-CAF; Walker-CV; Snawder-JE
Polycycl Aromat Compd 2011 Aug; 31(4):270-285
An enzyme-linked immunosorbent assay (ELISA) kit made for determination of polycyclic aromatic compounds (PACs) in water was adapted for measuring PACs and their metabolites in urine. This method was then applied to a pilot asphalt worker PAC exposure study. Currently, liquid-liquid extraction with gas chromatography/isotope dilution high-resolution mass spectrometry (GC/HRMS) is the preferred method to determine urinary PAC metabolites. Although sensitive and specific, GC/HRMS is time consuming and costly. The ELISA method had a range from 14-720 ng/ml 1-hydroxypyrene equivalents with a lower limit of detection (LOD) of 14 ng/ml urine. ELISA and GC/HRMS PAC metabolite measurements had a statistically significant correlation and the PAC ELISA results were indicative of potential asphalt exposure. PAC ELISA is promising as a more rapid and less costly routine method for determining worker exposure to PACs in asphalt emissions.
Analytical-processes; Asphalt-fumes; Asphalt-industry; Biological-effects; Biological-factors; Biological-monitoring; Biological-systems; Biomarkers; Chemical-hypersensitivity; Chemical-properties; Ethylenes; Exposure-assessment; Immune-reaction; Immune-system; Immunology; Inhalants; Lung-disorders; Lung-irritants; Metabolic-activation; Metabolic-study; Metabolites; Pulmonary-disorders; Pulmonary-system; Quantitative-analysis; Respiratory-hypersensitivity; Respiratory-irritants; Respiratory-system-disorders; Statistical-analysis; Urine-chemistry; Work-areas; Worker-health; Work-operations; Work-performance; Workplace-studies;
Author Keywords: asphalt; biomonitoring; ELISA; PAC; urine
Jerome P. Smith, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, 4676 Columbia Pkwy C-26, Cincinnati, OH 45226
Polycyclic Aromatic Compounds