Production, characterization and utility of a panel of monoclonal antibodies 2 for the detection of toluene diisocyanate haptenated proteins.
Ruwona-TB; Johnson-VJ; Hettick-JM; Schmechel-D; Beezhold-D; Wang-W; Simoyi-RH; Siegel-PD
J Immunol Methods 2011 Oct; 373(1-2):127-135
Diisocyanates (dNCOs) are highly reactive low molecular weight chemicals used in the manufacture of polyurethane products and are the most commonly reported cause of occupational asthma. Mechanistic disease studies and development of biomonitoring and research tools, such as monoclonal antibodies (mAbs) have been hampered by dNCOs' ability to self- polymerize and to cross-link biomolecules. Toluene diisocyanate (TDI)-specific monoclonal antibodies (mAbs), with potential use in immunoassays for exposure and biomarker assessments, were produced and reactivities characterized against mono- and diisocyanate and dithioisocyanate protein conjugates. In general, TDI reactive mAbs displayed stronger recognition of isocyanate haptenated proteins when the NCO was in the ortho position relative to the tolyl group, and were capable of discriminating between isocyanate and isothiocyanate conjugates and between aromatic and aliphatic dNCOs. Preliminary studies using TDI vapor exposed cells suggest potential utility of these mAbs for both research and biomonitoring.
Biological-agents; Biological-effects; Biological-monitoring; Biological-systems; Exposure-assessment; Immunologic-disorders; Immunology; Immunotoxins; Lung-cells; Lung-disorders; Lung-function; Pulmonary-system; Pulmonary-system-disorders; Respiratory-hypersensitivity; Respiratory-irritants; Respiratory-system-disorders; Work-environment; Workplace-monitoring; Workplace-studies;
Author Keywords: TDI; Monoclonal antibody; Occupational asthma
Paul D. Siegel, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505-2888
Healthcare and Social Assistance; Services
Journal of Immunological Methods