Competitive immunochromatographic assay for the detection of the organophosphorus pesticide chlorpyrifos.
Kim-YA; Lee-E-H; Kim-K-O; Lee-YT; Hammock-BD; Lee-H-S
Anal Chim Acta 2011 May; 693(1-2):106-113
An immunochromatographic assay (ICA) based on competitive antigen-coated format using colloidal gold as the label was developed for the detection of the organophosphorus insecticide chlorpyrifos. The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with chlorpyrifos Hapten-OVA conjugate (test line) and anti-mouse IgG (control line). Based on the fact that the competition is between the migrating analyte in the sample and the analyte hapten immobilized on the test strip for the binding sites of the antibody-colloidal gold (Ab-CG) conjugate migrating on the test strip, this study suggests that the relative migration speed between the two migrating substances is a critically important factor for the sensitive detection by competitive ICA. This criterion was utilized for the confirmation of appropriateness of a nitrocellulose (NC) membrane for chlorpyrifos ICA. The detection limit of the ICA for chlorpyrifos standard and chlorpyrifos spiked into agricultural samples were 10 and 50 ng mL(-1), respectively. The assay time for the ICA test was less than 10 min, suitable for rapid on-site testing of chlorpyrifos.
Antibody-response; Bioassays; Environmental-health-monitoring; Exposure-assessment; Immunological-tests; Insecticides; Laboratory-animals; Laboratory-techniques; Laboratory-testing; Organo-phosphorus-pesticides; Pesticides; Pesticides-and-agricultural-chemicals;
Author Keywords: Organophosphorus pesticide; Chlorpyrifos; Immunochromatographic assay
Hye-Sung Lee, Department of Food Science and Nutrition, Kyungpook National University, Daegu 702-701, Republic of Korea
Agriculture; Cooperative Agreement
Agriculture, Forestry and Fishing
Analytica Chimica Acta
University of California - Davis