Hemoglobin adducts in workers exposed to 1,6-hexamethylene diisocyanate.
Flack-SL; Fent-KW; Gaines-LGT; Thomasen-JM; Whittaker-SG; Ball-LM; Nylander-French-LA
Biomarkers 2011 May; 16(3):261-270
We investigated the utility of 1,6-hexamethylene diamine (HDA) hemoglobin adducts as biomarkers of exposure to 1,6-hexamethylene diisocyanate (HDI) monomer. Blood samples from 15 spray painters applying HDI-containing paint were analyzed for hemoglobin HDA (HDA-Hb) and N-acetyl-1,6-hexamethylene diamine (monoacetyl-HDA-Hb) by GC-MS. HDA-Hb was detected in the majority of workers (=1.2-37ng/g Hb), whereas monoacetyl-HDA-Hb was detected in one worker (0.06 ng/g Hb). The stronger, positive association between HDA-Hb and cumulative HDI exposure (r(2)=0.3, p<.06) than same day exposure (p>/=0.13) indicates long-term elimination kinetics for HDA-Hb adducts. This association demonstrates the suitability of HDA-Hb adducts for further validation as a biomarker of HDI exposure.
Airborne-particles; Biological-effects; Chemical-hypersensitivity; Dermatitis; Exposure-assessment; Inhalants; Inhalation-studies; Paint-spraying; Particle-aerodynamics; Particulates; Quantitative-analysis; Respiratory-hypersensitivity; Respiratory-irritants; Statistical-analysis; Biomarkers;
Author Keywords: 1,6-hexamethylene diamine (HDA); hemoglobin; adduct; biomarker; 1,6-hexamethylene diisocyanate (HDI)
Leena A. Nylander-French, Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, CB #7431, Rosenau Hall, Chapel Hill, NC 27599-7431
Grant-Number-R01-OH-007598; Grant-Number-T42-CCT-422952; Grant-Number-R01-OH-009086; Grant-Number-T42-OH-008673
University of North Carolina, Chapel Hill, North Carolina