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Collection efficiencies of an electrostatic sampler with superhydrophobic surface for fungal bioaerosols.

Han T; Nazarenko Y; Lioy PJ; Mainelis G
Indoor Air 2011 Apr; 21(2):110-120
We recently developed an electrostatic precipitator with superhydrophobic surface (EPSS), which collects particles into a 10- to 40-microl water droplet allowing achievement of very high concentration rates (defined as the ratio of particle concentration in the collection liquid vs. the airborne particle concentration per time unit) when sampling airborne bacteria. Here, we analyzed the performance of this sampler when collecting three commonly found fungal spores--Cladosporium cladosporioides, Penicillium melinii, and Aspergillus versicolor--under different operating conditions. We also adapted adenosine triphosphate (ATP)-based bioluminescence for the analysis of collection efficiency and the concentration rates. The collection efficiency ranged from 10 to 36 percent at a sampling flow rate of 10 l/min when the airborne fungal spore concentration was approximately 10(5)-10(6) spores/m(3) resulting in concentration rates in the range of 1 × 10(5)-3 × 10(5)/min for a 10-microl droplet. The collection efficiency was inversely proportional to the airborne spore concentration and it increased to above 60 percent for common ambient spore concentrations, e.g., 10(4)-10(5) spores/m(3). The spore concentrations determined by the ATP-based method were not statistically different from those determined by microscopy and allowed us to analyze spore concentrations that were too low to be reliably detected by microscopy. PRACTICAL IMPLICATIONS: The new electrostatic precipitator with superhydrophobic surface (EPSS) collects airborne fungal spores into small water droplets (10 and 40 µl) allowing achievement of concentration rates that are higher than those of most currently available bioaerosol samplers. Biosamplers with high concentration rates enable detection of low ambient aerial bioaerosol concentrations in various environments, including indoors air, and would be useful for improved exposure assessment. A successful adaptation of the adenosine triphosphate (ATP)-based bioluminescence assay for the quantification of fungal spores from a specific species enables fast sample analysis in laboratory investigations. This rapid assay could be especially useful when investigating the performance of biological samplers as a function of multiple operational parameters.
Electrostatic-precipitators; Hydrophobic-bonds; Particulate-sampling-methods; Airborne-particles; Air-sampling; Air-sampling-techniques; Bacteria; Samplers; Sampling-equipment; Fungi; Analytical-instruments; Adenosines; Biological-material; Luminescence; Aerosol-particles; Aerosol-sampling; Aerosols; Surface-properties; Indoor-air-pollution; Microbiology; Microorganisms; Microscopic-analysis; Air-contamination; Air-quality-measurement; Air-purification; Author Keywords: Electrostatic precipitation; Bioaerosols; Fungi; Collection efficiency; Concentration rate; ATP bioluminescence
G. Mainelis Department of Environmental Sciences Rutgers University, 14 College Farm Rd New Brunswick, NJ 08901, USA
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Indoor Air
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Rutgers the State of New Jersey - New Brunswick
Page last reviewed: March 18, 2022
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