The LLNA: a brief review of recent advances and limitations.
Anderson-SE; Siegel-PD; Meade-BJ
J Allergy 2011 Jan; 2011:424203
Allergic contact dermatitis is the second most commonly reported occupational illness, accounting for 10 percent to 15 percent of all occupational diseases. This highlights the importance of developing rapid and sensitive methods for hazard identification of chemical sensitizers. The murine local lymph node assay (LLNA) was developed and validated for the identification of low molecular weight sensitizing chemicals. It provides several benefits over other tests for sensitization because it provides a quantitative endpoint, dose-responsive data, and allows for prediction of potency. However, there are also several concerns with this assay including: levels of false positive responses, variability due to vehicle, and predictivity. This report serves as a concise review which briefly summarizes the progress, advances and limitations of the assay over the last decade.
Allergens; Allergic-dermatitis; Allergic-disorders; Allergic-reactions; Allergies; Dermatitis; Dermatosis; Skin-diseases; Skin-disorders; Skin-exposure; Skin-irritants; Skin-lesions; Sensitivity-testing; Medical-monitoring; Medical-screening; Bioassays; Chemical-hypersensitivity; Chemical-reactions; Lymph-nodes; Molecular-biology; Molecular-structure; Quantitative-analysis; Dose-response
Stacey E. Anderson, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Drive, Morgantown, WV 26505
Journal of Allergy