Identification of systemic markers from a pulmonary carbon nanotube exposure.
Erdely-A; Liston-A; Salmen-Muniz-R; Hulderman-T; Young-S-H; Zeidler-Erdely-PC; Castranova-V; Simeonova-PP
J Occup Environ Med 2011 Jun; 53(6S):S80-S86
Objective: Interest exists for early monitoring of worker exposure to engineered nanomaterials. Here, we highlight quantitative systemic markers of early effects after carbon nanotube (CNT) exposure. Methods: Mice were exposed by pharyngeal aspiration to 40-microg CNT and harvested 24 hours, 7 days, and 28 days postexposure for measurements of whole blood, lung and extrapulmonary tissue gene expression, blood and bronchoalveolar lavage (BAL) differentials, and serum protein profiling. Results: Early effects included increased inflammatory blood gene expression and serum cytokines followed by an acute phase response (eg, CRP, SAA-1, SAP). Beyond 24 hours, there was a consistent increase in blood and BAL eosinophils. At 28 day, serum acute phase proteins with immune function including complement C3, apolipoproteins A-I and A-II, and a2-macroglobulin were increased. Conclusions: Carbon nanotube exposure resulted in measurable systemic markers but lacked specificity to distinguish from other pulmonary exposures.
Nanotechnology; Nanotubes; Toxicology; Hazardous-materials; Employee-exposure; Workplace-monitoring; Quantitative-analysis; Biomarkers; Medical-monitoring; Biological-effects; Animal-studies; Animals; Laboratory-animals; Laboratory-testing; Exposure-levels; Exposure-methods; Blood-analysis; Lung-tissue; Pulmonary-system; Tissue-culture; Genes; Alveolar-cells; Proteins; Cellular-reactions; Dose-response; Immune-reaction; Immunoglobulins; Pulmonary-system-disorders; Cytotoxic-effects
Aaron Erdely, PhD, NIOSH/HELD/TMBB, 1095 Willowdale Rd, MS-3014, Morgantown, WV 26505
Journal of Occupational and Environmental Medicine