Silver nanowires induced inflammation in an in vitro human alveolar lung model.
Schaeublin-NM; Estep-CA; Roberts-JR; Hussain-SM
Toxicologist 2011 Mar; 120(Suppl 2):468
The goal of this study was to evaluate the biological response following inhalation exposure to silver nanowires (Ag-NWs). Nanomaterials have been shown to penetrate deep into the alveolar regions of the lung, so we used a human alveolar lung co-culture model previously established in our lab as our model for inhalation exposure. The Ag-NWs were synthesized by nanoComposix and were 4 and 20 mu m in length, with a similar diameter of approximately 90 nm. Changes in cellular morphology and the cellular interaction of the Ag-NWs with the cells was assessed using ultra-resolution microscopy after a 24 h exposure to 200 ng/ml of the Ag-NWs. The cell morphology and interaction of the Ag-NWs demonstrated that both lengths of Ag-NWs were interacting with the cells, but normal morphology indicated that they were not toxic. In addition, the co-cultures were exposed to Ag-NWs (concentrations ranging from 0-200 ng/ml) and cell viability was assessed using MTS to evaluate mitochondrial function. Our viability assays indicated that both Ag-NWs did not alter cell viability and that there was no toxicity. In addition, inflammatory responses were evaluated using ELISA assays to determine the secretion of 12 different cytokines. Of the cytokines evaluated, the Ag-NWs demonstrated an increase in secretion of several of them including IL-6, IL-8 and Interferon Gamma indicating that the Ag-NWs caused inflammation. Therefore, while the Ag-NWs were not toxic to the cells, they did cause irritation and an inflammatory response.
Biological-effects; Cell-biology; Cell-morphology; Cytology; Laboratory-testing; Lung-irritants; Lung-tissue; Molecular-biology; Nanotechnology; Pulmonary-disorders; Quantitative-analysis; Respiratory-hypersensitivity; Respiratory-irritants; Statistical-analysis; Tissue-disorders
The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC