Currently there is expanding interest, from the perspectives of occupational health surveillance and future epidemiological research, in early monitoring of worker exposure to engineered nanomaterials. Here, we highlight quantitative systemic markers in mice after a single exposure to carbon nanotubes (CNT) (multi-walled (MW) CNT or single-walled (SW) CNT). Mice were exposed by pharyngeal aspiration to 40microg and harvested at 24 hr, 7 and 28d post exposure. Mice were sacrificed and the following parameters were analyzed: whole blood gene expression, blood and bronchoalveolar lavage (BAL) differentials, serum protein profiling by immunoplex and proteomics, and gene expression analysis in the lung and extrapulmonary tissues including aorta, heart and liver. Early effects of pulmonary CNT exposure include a rapid but transient increase in inflammatory blood gene expression (IL-1beta, CXCL2) and serum cytokines (IL-6, IL-5, CCL11). This was followed by an acute phase response including CRP, SAA-1, SAP and haptoglobin as shown by elevated serum protein levels and liver gene expression. Beyond 24hr there was a consistent increase in both blood and BAL eosinophils. These data correspond well with early increased serum IL-5 and CCL11 after MWCNT exposure. At 28d, serum acute phase proteins with immune function including complement C3, apolipoprotein A-I and A-II, alpha2-marcoglobulin, serotransferrin and liver carboxylesterase N were increased. The systemic markers correlated well with existing literature showing end-point measurements of pulmonary CNT exposure and adverse cardiovascular effects and enhanced allergic response.
Airborne-particles; Allergic-reactions; Biological-effects; Cardiopulmonary-system; Cardiopulmonary-system-disorders; Cardiovascular-disease; Cardiovascular-system; Cardiovascular-system-disorders; Cellular-reactions; Epidemiology; Exposure-levels; Genetic-factors; Inhalation-studies; Laboratory-animals; Lung-disorders; Lung-irritants; Lung-tissue; Nanotechnology; Physiological-effects; Pulmonary-disorders; Pulmonary-system; Pulmonary-system-disorders; Quantitative-analysis; Respiratory-hypersensitivity; Respiratory-irritants; Respiratory-system-disorders
The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC