Evaluation of a test method for the measurement of the urinary biomarkers S-benzylmercapturic acid and S-phenylmercapturic acid.
Toxicologist 2011 Mar; 120(Suppl 2):306
High-Performance Liquid Chromatography-Mass Spectrometric (HPLC-MS) conditions were developed for use to detect and quantitate urinary levels of S-benzylmercapturic acid (S-BMA) and S-phenylmercapturic acid (S-PMA). S-BMA and S-PMA are the metabolites from toluene and benzene, respectively, and these compounds are important for occupational health studies owing to their use as biomarkers of exposure. Toluene is used extensively as a solvent, and the health hazards of benzene exposure have been well established. The developed test method included the use of the deuterated analog of both analytes for use as internal standards. Spiked urine specimens were passed through solid-phase extraction cartridges; then the analytes were removed by acetone washes. The liquid volume was reduced by evaporation, and the dry extracts were reconstituted in mobile phase solvent for introduction into the liquid chromatograph. A triple quadrupole mass spectrometer (MS/MS) was used as detector with gradient reversed-phase HPLC conditions to measure the target analytes. Recovery experiments using 1, 2, 6, 8 and 30 ng/ml S-BMA and S-PMA fortified urine samples demonstrated good accuracy and precision. Recovery of both target analytes varied between average values of 99 to 110% of theory for the various spiked levels. Linearity of response was verified for concentrations of 0.5 to 50 ng/ml, and correlation coefficients of 0.98 or greater were obtained for all standard curves. This method offers a valid test for the determination of the urinary levels of both of these biomarkers of exposure as demonstrated by the accuracy and precision of the recovery studies. Disclaimers: Mention of company names and/or products does not constitute endorsement by the National Institute for Occupational Safety and Health (NIOSH). The findings and conclusions in this poster have not been formally disseminated by NIOSH and should not be construed to represent any agency determination or policy.
Biomarkers; Chromatographic-analysis; Mass-spectrometry; Metabolic-activation; Metabolites; Physiological-effects; Quantitative-analysis; Solvents; Solvent-vapors; Spectroscopes
Healthcare and Social Assistance; Services; Manufacturing
The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC