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Toxicity of dusts collected from South African mines: free-radical production and lipid peroxidation.
Vetten M; Vallyathan V; Leonard SS; Kenyon A; Gulumian M
Toxicol Lett 2010 Jul; 196(Suppl 1):S70-S71
Occupational exposure to mine dust can damage lung tissue and cause respiratory diseases. In this study, dusts from five South African mines were collected and their toxicities assessed. The samples were obtained from various workplaces within the mines. Electron spin resonance was performed to evaluate the ability of the samples to produce hydroxyl radicals in the presence of hydrogen peroxide. From these initial experiments, six samples were selected for further studies. The samples were incubated with a bronchial epithelial cell line to determine levels of hydroxyl radicals produced by the cells in response to the dust. In addition, levels of lipid peroxidation, as determined by the 8-isoprostane concentration in cell culture media, were measured. These experiments showed variations in the generation of free radicals between samples from the different mines and within the mines themselves. Levels of 8-isoprostane also varied between samples. This data indicates differences in toxicity of the various dust samples, warranting further investigations to correlate the composition and characteristics of the samples to their toxicity.
Dust-exposure; Dust-particles; Exposure-assessment; Mining-industry; Toxic-materials; Toxicology; Dusts; In-vitro-studies
Toxicology Letters. Abstracts of the XII International Congress of Toxicology
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