Circulating leukocytes as indicators of arylamine carcinogen exposure.
Toxicologist 1992 Feb; 12(1):189
Circulating white blood cells (WBC) are being examined as a biological monitor of exposure to arylamines and other carcinogens. DNA-carcinogen adduct formation in WBC may be a marker for the degree of in vivo exposure to carcinogens as well as an indicator of DNA adduct formation in target tissues. Controlled exposure of inbred mice to 2-aminofluorene (AF) followed by 32P-postlabeling and HPLC analysis of nucleotides from WBC and the carcinogen target tissues liver and urinary bladder was used to evaluate the relationship between carcinogen exposure and DNA adduct formation. At 3 hr after a 60 mg/kg i.p. dose of AF, WBC from 7 wk old male C57BL/6J mice was ,adducted to a level of 24 fmol/mg. The target tissues, liver and bladder, were adducted to 180 fmol/mg and 465 fmol/mg, respectively. The adduct level in DNA from all three tissues decreased with time such that at 24 hr post-exposure, WBC DNA adducts had decreased to 7 fmol/mg, while liver and -bladder DNA adducts decreased to 50 fmol/mg and 230 fmol/mg. Preliminary results thus indicate that after exposure to arylamine carcinogens WBC DNA is adducted with a time course similar to target tissues. Studies in progress relate carcinogen dose to adduct formation and consider sub-chronic exposure in addition to one time acute doses.
Arylamines; Blood-cells; Blood-analysis; Blood-tests; Carcinogens; Exposure-levels; Tissue-culture; Animal-studies; Animals; DNA-adducts; Dose-response
The Toxicologist. Society of Toxicology 31st Annual Meeting, February 23-27,1992, Seattle, Washingtion
University of Michigan at Ann Arbor, Ann Arbor, Michigan