In vitro lymphocyte responses in contact hypersensitivity III.
J Invest Dermatol 1972 Jun; 58(6):388-391
Guinea pigs were sensitized to one of two contact allergens: dinitrofluorobenzene (DNFH) or paraphenylenediamine (PDA). Sensitization was accomplished by footpad injections of the hapten conjugated to an extract of soluble guinea pig epidermal proteins in Freund's complete adjuvant. Lymphocytes from animals with contact hypersensitivity to DNFB or PDA were cultured in vitro with guinea pig epidermal proteins conjugated to DNFB or PDA. Responses to these antigens, as measured by incorporation of tritiated thymidine into DNA, indicated that lymphocyte transformation in vitro can distinguish between sensitivity to either of these allergens.
Allergic-dermatitis; Allergic-disorders; Allergic-reactions; Allergies; Antigens; Dermatitis; Dermatosis; Exposure-assessment; Exposure-levels; Exposure-methods; Hypersensitivity; Immunology; Laboratory-animals; Laboratory-testing; Skin-disorders; Skin-exposure; Skin-irritants; Skin-sensitivity
John E Milner, Department of Medicine, School of Medicine, and the Department of Environmental Health, School of Public Health, University of Washington, Seattle, Washington, 98195
Journal of Investigative Dermatology
University of Washington, Seattle, Washington