Tungsten carbide-cobalt nanoparticles induce reactive oxygen species, AKT, ERK, AP-1, NF-kB, VEGF, and angiogenesis.
Ding-M; Zhao-J; Bowman-L; Castranova-V; Jiang-B-H
FASEB J 2010 Apr; 24(Meeting Abstracts):833.7
Powder mixtures of tungsten carbide and metallic cobalt (WC-Co) are widely used in various products. Nanoparticles are engineered structures with at least one dimension of 100 nanometers or smaller. WC-Co is known to be associated with lung injury and diseases. However, the cellular effects of WC-Co remain to be elucidated. In this study, we showed that WC-Co nanoparticles at 5 ug/cm2 induced reactive oxygen species (ROS) production which activated AKT and ERK signal pathways in a lung epithelial cell line. ROS played a key role in WC-Co induced transcriptional activation of AP-1, NF-kB,VEGF, AKT and ERK1/2; while the fine size of WC-Co particles at the same concentration did not have much effect. However, at higher concentrations, fine-size WC-Co particles also induced ROS production, AKT and ERK1/2 activation. WC-Co nanoparticles also affected the cells to induce angiogenesis. These results identify multiple signaling molecules that are induced by WC-Co nanoparticle treatment, and elucidate the potential molecular mechanism of their regulation in the cells. This information may be useful for preventing potential damage from WC-Co particle exposure in the future.
Tungsten-compounds; Nanotechnology; Lung-disease; Lung-disorders; Respiratory-system-disorders; Pulmonary-system-disorders; Antioxidants
Issue of Publication
The FASEB Journal. Experimental Biology 2010, April 24 - 28, 2010, Anaheim, California