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Biomarkers and metabolites: HPLC/MS analysis.
Encyclopedia of chromatography, 3rd edition. Cazes J, ed. Boca Raton, FL: Taylor and Francis, 2009 Oct; 1:238-246
High-performance liquid chromatography-mass spectrometry (HPLC-MS) is a powerful analytical technique widely used in recent years for the analysis of biomarkers and metabolites. Biomarker determination and quantification, whether metabolic or adducted biomolecules, are commonly used to evaluate exposure and support biomonitoring research, especially in the area of occupational exposure and health. Some of the common problems and strategies of HPLC-MS biomarker analysis involve matrix effects, the use of isotope-labeled internal standard compounds, and sample cleanup; usually all of these factors must be evaluated within the development phase of an analysis procedure. Specific examples of biomarker analysis using HPLC-MS include acrylamide, aromatic compounds, and 1-bromopropane, and these examples are discussed in detail.
Analytical-processes; Analytical-chemistry; Analytical-methods; Biomarkers; Sampling-methods; Sample-preparation; Author Keywords: biomarkers; metabolites; acrylamide; aromatics; 1-bromopropane
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Encyclopedia of chromatography, 3rd edition
Page last reviewed: March 11, 2019
Content source: National Institute for Occupational Safety and Health Education and Information Division