NIOSHTIC-2 Publications Search

Biomarkers and metabolites: HPLC/MS analysis.

Authors
B'Hymer-C; Cheever-KL
Source
Encyclopedia of chromatography, 3rd edition. Cazes J, ed. Boca Raton, FL: Taylor and Francis, 2009 Oct; 1:238-246
NIOSHTIC No.
20036137
Abstract
High-performance liquid chromatography-mass spectrometry (HPLC-MS) is a powerful analytical technique widely used in recent years for the analysis of biomarkers and metabolites. Biomarker determination and quantification, whether metabolic or adducted biomolecules, are commonly used to evaluate exposure and support biomonitoring research, especially in the area of occupational exposure and health. Some of the common problems and strategies of HPLC-MS biomarker analysis involve matrix effects, the use of isotope-labeled internal standard compounds, and sample cleanup; usually all of these factors must be evaluated within the development phase of an analysis procedure. Specific examples of biomarker analysis using HPLC-MS include acrylamide, aromatic compounds, and 1-bromopropane, and these examples are discussed in detail.
Keywords
Analytical-processes; Analytical-chemistry; Analytical-methods; Biomarkers; Sampling-methods; Sample-preparation; Author Keywords: biomarkers; metabolites; acrylamide; aromatics; 1-bromopropane
CAS No.
79-06-1; 106-94-5
Publication Date
20091012
Document Type
Chapter
Editors
Cazes-J
Fiscal Year
2010
ISBN No.
9781420084597
NIOSH Division
DART
Priority Area
Services; Healthcare and Social Assistance
Source Name
Encyclopedia of chromatography, 3rd edition
State
OH
Page last reviewed: March 11, 2019
Content source: National Institute for Occupational Safety and Health Education and Information Division