Effect of oxygen radicals on surfactant activity.
Vallyathan-V; Green-FHY; Schurch-S; Leonard-S; Wallace-WE
FASEB J 1995 Mar; 9(4)(II):A973
Alterations in the surface properties of lung surfactant have been demonstrated in several acute occupational lung diseases. Oxygen radicals have been implicated in their pathogenesis. Oxygen radicals could cause peroxidation of surfactant lipids and denature proteins facilitating the disease process. The purpose of this study was to determine the effects of superoxide anion, hydroxy radical and hydrogen peroxide on the surface tension properties of bovine pulmonary surfactant and to correlate these changes with the formation of lipid peroxides. The surface tension measurements were made in a captive bubble surface tensiometer with and without the generation of oxygen radicals over time. The generation of oxygen radicals in identical systems was also monitored in an electron spin resonance spectrometer using a spin trap. Superoxide radicals induced a significant adsorption and surface tension effect on surfactant. H2O2 was found to be a potent inhibitor of surfactant activity, while H2O2 in the presence of iron was not inhibitory. The formation of lipid peroxides from surfactant by H2O2 was supportive of an inhibitory role of peroxides. In conclusion, the results show that oxygen radicals and other reactive intermediates can inhibit surfactant activity which may have pathophysiological significance in the disease process.
Biochemical-analysis; Biochemistry; Biological-effects; Biological-systems; Cell-biology; Cell-function; Cellular-reactions; Chemical-hypersensitivity; Chemical-reactions; Exposure-assessment; Exposure-levels; Exposure-methods; Inhalation-studies; Lung; Lung-disease; Lung-disorders; Lung-irritants; Pathogenesis; Pathogens; Physiological-effects; Physiological-factors; Physiological-response; Pulmonary-disorders; Pulmonary-system-disorders; Surface-properties
Abstract; Conference/Symposia Proceedings
The FASEB Journal. Experimental Biology 95 - Annual Meeting of Professional Research Scientists, Atlanta, Georgia, April 9-13, 1995