Exposure to crystalline silica or treatment with chlorphentermine increases alveolar-lavage vitamin-E levels.
Miles-PR; Bowman-L; Reasor-MJ
FASEB J 1995 Mar; 9(4)(II):A717
Vitamin E may be an integral part of lung surfactant (Am. J. Physiol. 265:L133-L139, 1993). Therefore, we measured vitamin E levels in alveolar lavage materials from rats exposed to silica or treated with chlorphentermine (CP), two treatments which are known to increase surfactant phospholipids (PL) by different mechanisms. Silica exposure leads to increased PL synthesis, and CP treatment causes reduced PL degradation. HCI-washed and unwashed silica were used because exposure to each of them leads to different degrees of phospholipidosis. Exposure to HCI-washed silica results in a 17-fold increase in lavage PL and protein levels and a 12.2-fold increase in the amount of vitamin E. Exposure to unwashed silica leads to a 7-fold increase in PL and proteins and a 5.8-fold increase in lavage vitamin E. Following treatment of rats with CP, there are 15- to 19-fold increases in lavage PL and proteins and a 13.6-fold increase in vitamin E. Because surfactant synthesis occurs in the endoplasmic reticulum, we also measured vitamin E in lung microsomes. Both silica exposure and CP treatment lead to 1.8- to 2.5-fold increases in the lung microsomal levels of vitamin E. These results demonstrate that alveolar lavage vitamin E levels are elevated along with lavage PL and proteins and lung microsomal vitamin E levels are increased following exposure of rats to silica or treatment of the animals with CP.
Biochemical-analysis; Biochemistry; Biological-effects; Biological-systems; Cell-biology; Cell-function; Cellular-reactions; Exposure-assessment; Exposure-methods; Inhalation-studies; Laboratory-animals; Laboratory-techniques; Laboratory-testing; Lung-function; Lung-irritants; Molecular-biology; Molecular-structure; Pulmonary-system; Silicates; Silicon-compounds
Abstract; Conference/Symposia Proceedings
The FASEB Journal. Experimental Biology 95 - Annual Meeting of Professional Research Scientists, Atlanta, Georgia, April 9-13, 1995